![]() ![]() Our results suggest that at least two types of islet-specific CD4 + T-cell clones play a role in diabetes pathogenesis. ![]() One such class of vectors includes the Invitrogen TOPO cloning vectors which contain covalently linked DNA topoisomerase I that functions. In this case the cDNA has been incorporated into a vector in a position where it is within a coding sequence of another protein. To further simplify and streamline the cloning workflow, specialized vectors have been developed to place an insert into vector, for example, without using a ligase. NOD-scid recipients of cotransferred BDC-2.5/CD8 + splenic T-cells showed a small population of CD4 + T-cells and a larger population of CD8 + T-cells within the infiltrated islets, whereas no infiltrate was detectable in recipients of CD8 + splenocytes or BDC-2.5 alone. In many cases a vector can be designed so that the cDNA will be expressed, frequently as a fusion protein. In contrast, NOD- scid mice that received BDC-6.9 showed only the presence of CD4 +Vβ4 + T-cells and a large population of MAC-1 + cells in islet lesions. Immunohistochemical staining of pancreatic lesions in young NOD mice receiving either BDC-2.5 or BDC-6.9 showed the presence of CD4 +, CD8 +, Vβ4 +, and MAC-1 + cells within the infiltrate, similar to infiltrates in lesions of spontaneously diabetic female NOD mice. Even though BDC-2.5 by itself readily induces diabetes in young unmanipulated NOD mice, cotransfer of CD8-enriched T-cells was required to induce disease in NOD-scid mice. ![]() Try contacting the website administrator for help. The most likely reason is that the file no longer exists, so there is nothing for Ruffle to load. Severe combined immunodeficiency, or SCID, is a term applied to a group of inherited disorders characterized by defects in both T and B cell responses, hence the term 'combined. Biotechnology has been used for improving livestock and crops since the beginning of agriculture through selective breeding. Biotechnology is the use of artificial methods to modify the genetic material of living organisms or cells to produce novel compounds or to perform new functions. However, a second islet-specific T-cell clone, BDC-2.5, showing the same cytokine profile and T-cell receptor Vβ expression as BDC-6.9 was not capable of inducing diabetes or insulitis in NOD-scid mice. Ruffle failed to load the Flash SWF file. The antibodies attack foreign substances, or antigens, that mark invading viruses, bacteria and fungi. Explain molecular and reproductive cloning. Transfer of an interleukin 2/interferon-γ-secreting islet-specific CD4 + T-cell clone, BDC-6.9, in the immunodeficient NOD-scid mouse induces destruction of pancreatic β-cells without help from host B-cells, CD4 + T-cells, or CD8 + T-cells. ![]()
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![]() Please be aware that this starts a ~200MB download.Īlternatively, you can turn on the auto-install feature and let MiKTeX install missing packages on-the-fly. If you have just installed MiKTeX, then you have the option to upgrade your installation to a standard TeX system. Or, if you have a shared installation: sudo initexmf -admin -set-config-value AutoInstall=1 ![]() You also may want to enable automatic package installation: initexmf -set-config-value AutoInstall=1 Executables like lualatex will be installed in /usr/local/bin. To finish with a shared (system-wide) TeX installation. Executables like lualatex will be installed in ~/bin. To finish with a private (for you only) TeX installation. If this is the case and if you expect that some of your users would like to work with MiKTeX, then you should install MiKTeX system-wide.Ĭlick one of the two buttons if you have made your decision. The second option makes sense if you are the administrator of a multi-user system. You probably want to choose the first option. Search&click the MiKTeX Console icon to start MiKTeX Console:ĭo you want to set up a private (for you only) TeX installation, or do you want to set up a shared (system-wide) TeX installation? You can use MiKTeX Console or, if you prefer the command line, miktexsetup. Register installation source sudo curl -L -o /etc/zypp/repos.d/miktex.repo īefore you can use MiKTeX, you have to finish the setup. Register installation source Fedora 30 sudo curl -L -o /etc//miktex.repo įedora 28 sudo curl -L -o /etc//miktex.repo Register installation source echo "deb stretch universe" | sudo tee /etc/apt//miktex.listįedora 1. Ubuntu 16.04, Linux Mint 18.3 echo "deb xenial universe" | sudo tee /etc/apt//miktex.listĭebian 9 1. Register installation source Ubuntu 18.04, Linux Mint 19 echo "deb bionic universe" | sudo tee /etc/apt//miktex.list Use the package management system to install MiKTeX.Register the installation source which contains the MiKTeX installation package.Register the GPG key with which MiKTeX installation packages and metadata is signed. ![]() Installing MiKTeX on Linux involves these steps: *Note for both Windows and Mac, it is recommended that you set up a private installation (only for you) when prompted in the Wizard setup.ĥ) Once the installation is complete, you may want to check for updates.Ħ) This process will install the MikTeX console, which you can use to check for updates, upload new packages and change settings, if needed.ħa) From here you can open the TeXworks editor, which is where you will do your work.ħb) You can also open the TeXworks editor from your apps list or by searching for it.Ĩ) Depending on how you configured your installation, if you call a package that is not installed, you may be prompted to install it before your document can be compiled.MiKTeX is available for selected Linux distributions. ![]() The Windows download file is approximately 128MB and the Mac file is approximately 58MB.ġ) Navigate to the MikTeX downloads page and select your operating system from the tabs.ģ) Once the file has downloaded, open it to start the installation wizard.Ĥ) For a step by step tutorial on setting up the installation, please consult the following tutorials: It is recommended that you download the "Installer" version as this will walk you through the installation step by step. ![]() MikTeX can be installed on Windows, Mac and Linux machines. To work with LaTeX on your local computer, you can download and install an open source program called MikTeX. ![]() ![]() Sometimes, the slope of a line may be expressed in terms of tangent angle such as: m tan. This invariance of slope differences makes slope an angular invariant measure, on a par with circular angle (invariant under rotation) and hyperbolic angle, with invariance group of squeeze mappings. We can write the formula for the slope-intercept form of the equation of line L whose slope is m and x-intercept d as: y m (x d) Here, m Slope of the line. Slope: m = Δ y Δ x = tan ( θ ) of slopes is the same before and after the shear. ![]() For other uses, see Slope (disambiguation). Step 1: Enter the linear equation you want to find the slope and y-intercept for into the editor. For slope of a physical feature, see Grade (slope). In the slope-intercept form you use the slope of the line and the y-intercept to express the linear function. ![]() ![]() Now were going to focus on the slope-intercept form y mx + b. Earlier in this chapter we have expressed linear equations using the standard form Ax + By C and also y mx +b. This article is about the mathematical term. Functions Inverse Calculator Full pad Examples Frequently Asked Questions (FAQ) How do you calculate the inverse of a function To calculate the inverse of a function, swap the x and y variables then solve for y in terms of x. The slope-intercept form of a linear equation. ![]() ![]() ![]() It allows you to run multiple instances of Mini Heroes simultaneously, enabling you to play multiple accounts at once or play different games side-by-side. Multi-instance mode is another feature that makes BlueStacks stand out. It can help you to customize your controls according to your preferences, allowing you to move your heroes around more efficiently, cast spells and attacks with ease, and switch between your heroes quickly. Additionally, BlueStacks’ keymapping feature enables you to map game controls onto your keyboard or gamepad, making it easier to play the game. Additionally, playing on a PC can reduce the strain on your eyes and help prevent eye fatigue, which can be a common issue when playing games on mobile devices. It allows you to view the game’s graphics and details more clearly, making it easier to navigate through the game’s various features and options. One of the most significant advantages of playing Mini Heroes on PC with BlueStacks is the larger screen size. BlueStacks offers tools such as keymapping, multi-instance mode, and gamepad support, among others, that can help you to customize your gameplay experience. With BlueStacks, you can take the gaming experience to the next level by using its advanced features. Playing Mini Heroes on a larger screen with better graphics and smoother controls can be a game-changer. Get ready to unleash your summoning powers and embark on an unforgettable adventure with Mini Heroes: Summoners War on BlueStacks! Enhance Your Gaming Experience Join us as we explore the enchanting world of Mini Heroes: Summoners War and discover how BlueStacks enhances the PC gaming experience. From its charming art style and humorous elements to its thrilling storylines and captivating gameplay modes, Mini Heroes: Summoners War has something for every player. In this article, we will delve into the exciting features that make this game a must-play. With the release date approaching, the excitement for Mini Heroes: Summoners War continues to build. With BlueStacks, you can enjoy a smooth and lag-free gaming experience, ensuring that your strategies are executed flawlessly. From engaging in challenging PvE encounters to clashing with other players in intense PvP matches and participating in legion battles, the game offers a diverse range of gameplay modes to suit different playstyles. Strategy plays a pivotal role in Mini Heroes: Summoners War, as you customize your squad, experiment with various hero combinations, and uncover synergies to gain an edge in battles. Our Android app player provides a seamless platform that enhances controls and visuals, elevating your gameplay to new heights. And now, with BlueStacks, you can take your gaming experience to the next level by playing Mini Heroes: Summoners War on your PC with BlueStacks. We’re on the edge of our seats with excitement, and we can’t wait to unveil even more thrilling updates and details in the next Developer’s Note.What sets Mini Heroes: Summoners War apart is its effortless adventure experience, enabling players to dive into the action anytime, anywhere. Take advantage of the ability to play on a bigger screen, use keyboard shortcuts for quicker actions, and enjoy a smoother performance to elevate your gameplay. With its seamless gameplay and enhanced graphics, BlueStacks allows you to immerse yourself in the world of Summoners War like never before. So, brace yourselves, Summoners, and prepare to welcome the formidable and into your arsenal.įor those who are looking to enhance their Summoners War experience even further, BlueStacks provides the perfect platform. The details shared herein are set to launch with the v8.1.2 update, ushering in a new era of strategic possibilities and monstrous power. Make sure to check out the Fusion Hexagram post-update and embrace the new possibilities that await. This new Fusion Recipe is your ticket to unlocking unparalleled power and adding a significant boost to your lineup. ![]() 4★ (Fire) Dokkaebi Princess and Sapsaree.Decoding the New Fusion RecipeĮager to welcome these new forces to your team? Here’s everything you need to know about assembling these powerful allies through the new Fusion Recipe: ![]() The potential for strategic gameplay and versatility that these new Monsters bring to the table is immense, and we are eagerly anticipating the myriad of ways in which you, our esteemed Summoners, will utilize them in your journey. In moments of peril, Sapsaree rises to the occasion, fighting valiantly to protect the princess, especially when she’s under inability effects. ![]() Geometric Rotation DefinitionĪ geometric rotation is a transformation that rotates an object or function about a given, fixed point in the plane at a given angle in a given direction. The location of the endpoint of this new segment is the rotation of the key point that is the endpoint of the original line segment. Then, orient the copied segment to form the given angle in the given direction with the original line segment. Next, for each of these line segments, create a new segment of equal length such that one endpoint of the new segment is the point of rotation. Then, draw a line segment from each of the key points to the point of rotation. How to Do Rotations in GeometryĪs with other transformations, begin by finding the key points’ coordinates in the given function or object. The point of rotation may be a vertex of a given object or its center in other situations. The most common point of rotation is the origin (0, 0). This measure can be given in degrees or radians, and the direction - clockwise or counterclockwise - is specified. ![]() ![]() The geometric object or function then rotates around this given point by a given angle measure. The angle of rotation will always be specified as clockwise or counterclockwise.īefore continuing, make sure to review geometric transformations and coordinate geometry.Ī rotation in geometry is a transformation that has one fixed point. The given point can be anywhere in the plane, even on the given object. ![]() Rotation in Geometry - Examples and ExplanationĪ rotation in geometry moves a given object around a given point at a given angle. ![]() ![]() Want to quickly make memes? Try the following best free meme generators! #1. It is undoubtedly a great meme generator app.10 Best Meme Generators to Generate Memes MemeChat has clearly uplifted the Meme Culture in India. Well money is not only the best thing on MemeChat, it also has a variety of Indian templates taken from different shows, movies and sometimes from real life incidents too. You just have to use their paid templates and make something creative with it, if your meme gets approved you will get real cash. If I say a meme maker app is providing money for making memes it will sound like a joke right? But it is not a joke it is reality, MemeChat is an Indian meme generating app which gives you real money for making memes. ![]() You can share all your creations directly with your friends on Mematic via whatsapp, messenger, Instagram etc. Create collages with the new layout tools and merge your photos into beautiful frames Use your own images or access a large variety of templates directly from the app. You can add funny captions to popular images or your own photos, add captions to images. It is an all in one application where you can place bets on many sports here on picsart you can do multiple work at one platform. However Picsart is not a meme generator app in particular but it is the most common app used by big Meme Creators it offers great kind of features like sticker maker, dress editor, video editor, png maker and a lot more. It offers around 600+ different meme templates and you can also merge one template with other and make more funny memes. You will get the app easily on playstore or you can just download it from google you will find Meme creator app on any browser. If we talk about the easiest meme maker apps, then this app comes on the top, it is the most sorted meme generating app in the market right now. The most amazing part about Meme Generator is its png editor you can cut any part of the image very easily and smoothly. The app is designed by ZomboDroid and is available for both premium and free users. You can use your personal images from the gallery too. Meme generator is one of the most oldest meme maker apps in the meme industry with more than 10M+ downloads on the google play store, it has about 1000+ free meme templates which can be used for making creative and sarcastic memes for social media and for personal use also. Read Also: Best Android Games Here a List of Top 5 Meme Maker Apps ![]() Well there are a number of different meme maker apps available for making memes, these meme generator apps are available on the Google Play Store, you just have to download them and start editing. You all understand what memes are but do you understand the procedures of making it? How to edit the faces, how to make png, how to write text and a lot of other things. ![]() Meme Marketing is used by various companies nowadays to advertise their products in a better and beneficial way. Many cricketers like Wasim Jaffer, Virender Sehwag use these memes to troll other International Cricketers. “Who controls the memes, controls the universe” this line is quoted from a tweet by Elon Musk well he is absolutely right, memes are the most important thing on the social media right now, we can see it on almost every social media platform, memes provide a new way to say our things sarcastically. ![]() ![]() Intimacy can’t be achieved without honesty and vulnerability.ĭo you observe yourself making more “I” statements or “You” statements? Needsīefore recovery, I never knew I had needs. Each I-statement represents something of a risk, an experiment of vulnerability. I’m uncomfortable vs You shouldn’t do thatĪnd apparently I-statements are also a way to achieve emotional intimacy.īecause “I-statements reveal the speaker, since it brings him/her out of hiding. So in recovery, we learn to use “I statements” to express for Me, Myself & I ONLY. “You should do this… Why are you doing it this way?… Have you done it (in the way I want) yet?” - Familiar? We want to exert influence to manipulate others to do what WE want them to. We seem to forget everyone has the permission to think, feel and react however they choose. In CoDA, I’ve learned to speak for myself.Īs Codependents, we tend to want to control how other people think, feel and react. I’ve also learnt to identify my feelings and express how I feel to people around me.ĭo you know how you’re feeling right now? In my recovery, I’ve learnt that feelings are important and all feelings are valid. How you respond to your feelings is what you can control. Steve writes in his book, Monkeytraps: Feelings cannot and should not be controlled. Last thing, feelings can’t be “controlled”. ![]() We need to learn to express our emotions/feelings in healthy ways. You know the people who hold it all in and then one day they outburst because they can’t hold it in anymore? That’s because they don’t know that feelings need to be expressed in a healthy manner which doesn’t end up being harmful to themselves and others. We need to learn to process how we are feeling. Hiding, pretending, simply stuffing your feelings or shutting them out doesn’t make you a better person. Or maybe, they’re not equipped to deal with their own feelings. Feeling one’s feelings may be a painful process, and people would rather avoid pain and pretend to be fine. People avoid feeling their feelings because they’re afraid of being real with themselves. Because this may mean they are “wrong” for being angry or appear weak if they show sadness. Therefore people are afraid to show that they are sad, or angry. “Do Not Be Angry, It Is Bad For You”, “Do Not Be Sad, Be Strong” or whatever other bullsh*t. There is too much shaming of “negative” emotions in society and Social Media. I never knew I need to honour my feelings, no matter WHAT I’m feeling. Not knowing what you are feeling is a characteristic of Codependency. I never knew I had the right to feel whatever I did. In the past I actually had to ask my husb “what/how” I should feel toward something or someone. I never knew the necessity of identifying them-what am I feeling: Glad, Mad, Sad or Scared? Also, we can tend to overstep other people’s boundaries by giving unsolicited advice or thinking we know better.ĭo you know what your boundaries are and are you firmly standing by them? Feelings So it’s not really in our social consciousness. As a Codependent, we’re really bad at deciding on a boundary and sticking to it, since we tend to be people-pleasers. She also used to not knock on our door before entering our bedroom, because she said she’s our mother and has every right to do whatever she wants to her children. She used to invade the privacy of her children by looking through our letters and our journals. Hah! When I think back about it, the person that “taught” me about boundaries, or lack thereof, was my mom. It sure felt like I’ve been going to meetings longer than that! What I’ve learned at Codependents Anonymous in a yearĬlick on any of the following to jump to its segment. ![]() I went for my first CoDA meeting on Thursday, and shared for the first time at my second on Thur, 3 Dec. I need to OWN my recovery.” So I went to check my emails - because I know I emailed my therapist, Steve, and group therapy mates after I went for my first CoDA meeting.Īfter digging through my emails, I finally found it. I NEED to know when I started, so that I can chart my progress. So what makes this so different?īut then one day, I decided, “ NO, I need to take charge of my recovery. I”m not the person who thinks: 2016 has been a good/bad year for me, etc. Initially I thought, “Who cares I’m not someone who remembers milestone years anyway”, I don’t keep track of which year I graduated, which year this or that. Every time at a meeting, during the call for CoDA birthdays, I would wonder when mine was. I didn’t know when I started going for meetings. I celebrated my one year with CoDA in early December □ A little back story: Owning my recovery I really like the message on each one: To Thine Own Self Be True. I celebrated my first year in Codependents AnonymousĪt Codependents Anonymous (CoDA) meetings, “CoDA birthdays” are celebrated and marked with chips. ![]() ![]() ![]() Otherwise I would have to pay thousands for individual fonts - which is not economical or smart," says Ted. Therefore I had to sign up for a subscription to Sky Fonts in order to get the fonts I was wanting to use. "This template was more complicated to build because I used fonts that would actually be used in a newspaper. This template can be purchased at: (Photoshop-Template)Īfter building those three templates, Ted decided it was time to develop a modern looking, broadsheet template. The images use smart object so you just double click the smart object layer to replace the images on the template." This template allows you to easily change the text and images. "It is easy to manipulate this template since many people have Adobe Photoshop and are familiar with how it works. They were! The template was place on Creative Market and has recently started picking up sales. Ted then decided to develop a template for Adobe Photoshop - just the front page - to see if people were interested in using it as a flyer. The tabloid template is also available at: People like the tabloid size because it easily fits in your hands whereas the broadsheet can be unwieldy," says Ted. "The tabloid size is becoming a close competitor to the traditional broadsheet newspapers. Ted used this service a few times and then decided to have his newspaper mass produced. ![]() Ted found a company called "Newspaper Club" that would print from a press and ship the newspaper to your door. The next template designed by Ted had correct sizing - and it printed on an actual printing press. Although it has the wrong dimensions, people still buy it because they can use it for personal projects. Ted put this template up for sale and was surprised by it's success. The template itself was initially designed with the wrong dimensions. "So I went online and printed out the New York Times and designed a template based off that." I looked through them and decided that none were of the design quality needed for a newspaper," says Ted. "There were very few template options available. Ted thought it would be easy to find a template for a newspaper online. Ted Fuller was approached by a client to develop a newspaper - something he had never done before. Marys, ON - ( SBWIRE) - A graphic designer from Canada has developed an old fashioned newspaper template inspired by the New York Times for Adobe InDesign. If you are not fully satisfied with the 7 newspaper style templates contact us for a full refund.St. 4 Page Letter Size Newspaper Template for Microsoft Word.1 Page Letter Size Newspaper Template for Microsoft Word.1 Page A3 Size (11x17) Template for Adobe Illustrator.1 Page Ledger Size Newspaper Template for Adobe Illustrator.The 4 page and 1 page Word templates use 100% free fontsġ2 Page Tabloid Newspaper Template for Adobe InDesignġ2 Page Broadsheet Newspaper Template for Adobe InDesignġ Page Letter & Ledger Newspaper Template Bundle for Adobe InDesignġ Page Ledger Size Newspaper Template for Adobe Photoshop.The Tea Times templates uses 100% free fonts and images.Prints at any size without losing quality (vector based).Prints on any printer and actual printing presses.A mix of templates for Adobe Illustrator, Photoshop, and InDesign, and Microsoft Word.Easily change the newspaper name, headlines, photos, articles, and other text throughout.Some Important Features of the Newspaper Templates: Please check before purchasing what program works with the template you want. Each template works in only one of the following programs: Microsoft Word, Photoshop, InDesign, Illustrator. All templates can be printed at home at any size without losing quality since they are vector based. The 11x15 template is printable by (an actual printing press). This bundle of 7 old style and modern newspaper templates and flyers are for news, events, announcements, and general use. ![]() ![]() Weave a cozy rug from old T-shirts using a simple DIY loom! Tutorial here. Paint watercolor flowers! Here’s a step by step tutorial to paint beautiful watercolor flowers in 20 minutes! If you have trouble downloading, send this post to friends so they can download and email to you! (Due to thousands of downloads happening on this site, we can’t personally email files, hope you understand! □ ) Isn’t the new year a time for new adventures? A couple of fun projects to consider for 2018! To download the 3D 2018 calendar template, click here for the colored version, and here for the black and white version.īring up the pdf, and right click “save” to save. Here it is! A one-of-a-kind 3D 2018 calendar perfect for your desk! Below is a photo from an earlier version, which shows the final step. The key is to pay attention when you glue the tabs: I found it easier to assemble the 2 halves, but leave a couple of the tabs loose, then glue the 2 halves together, finish with gluing the last few tabs. Start with the first half, glue each tab to the back side of the adjacent pentagon, and form half of the dodecahedron.įor the second half, do the same but leave a few of the tabs open, then glue the 2 halves together. Cut along the outer lines, score around each pentagon shape.
![]() You can also reach out to the company directly for some examples. Some memorial websites allow you to browse through their memorials to get a better idea of what their obituaries look like. Here are some questions you might want to ask when looking for the right website: There are a few things to consider when choosing which website you’d like to host your obituary. Things to consider when choosing an online optionĭue to the average cost of a printed obituary being so high, many have turned to online sources as a place to post obituaries. Now that social media is the way in which most people connect and communicate with their communities, printing a traditional obituary is no longer a necessity and is often times not as effective as sharing news online. Since newspapers were the way in which most people received any type of news, printing obituaries in the paper was also an extremely effective way to spread news before the internet. Obituaries were traditionally posted in the local newspaper as a way to alert the community of someone’s passing. Online obituaries, such as the free ones you can create here on Ever Loved, can vary in price, but are generally much cheaper than printed obituaries. In short, obituaries are often expensive due to the actual cost of printing and the fact that there used to be very few alternatives. ![]() If they offer online obituaries, they might include ads or links to other news stories that they can generate revenue off of. In an effort to stay afloat, many newspapers charge by the line or word for any printed obituaries they publish. Newspapers are forced to turn from traditional print to online methods as more and more people turn to digital outlets to get their news. According to, the total number of circulating newspapers (online included) was 28.5 million in 2018, down from 62.5 million in 1986. With the rise and everyday necessity of the internet, newspapers have slowly but surely been losing out on subscribers. Local papers were essential, alerting everyone of important news and updates that affected the local community. Traditionally, obituaries that were posted in the newspaper were often times the best way to spread the news that someone had passed. Many sites allow you to browse memorials that have recently been posted which can give you a good idea for what yours will look like once it’s up.Ĭreate a memorial website Why are obituaries so expensive? If you choose to post an online obituary, try to do some research before posting. Some of these websites even have pop-up ads that interrupt the reader as they read through an obituary. The downside with some online obituaries is that many websites (including those hosted by newspapers) will clutter the obituary page with ads (something we do not do). Many newspapers still charge for this (generally around $50), but you can publish an obituary for free on Ever Loved. For reference, a traditional obituary typically has around 700 characters in it, which can rack up the costs of a printed obituary real quick.īecause newspaper obituaries are so expensive and fewer people read a physical paper, more and more people are publishing obituaries online. Each line is written in size 7 Serif font (a notoriously small font) and is only 28 characters long. ![]() For example, the New York Times charges $236 for the first four lines and $50 per line after that. The size and reach of the newspaper can also determine how expensive the obituary may be. The average cost of an obituary is generally between $200-$500 and that usually does not include a photo. ![]() Traditional obituaries that are posted in the newspaper are much more expensive than most people expect them to be. Purchase from another business, we may receive payment. ![]() |
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